Effect of chromium and L-carnosine use on oxidative stress markers in experimental model of gestational diabetes mellitus
Chromium and L-carnosine in GDM
Keywords:
Experimental diabetes, pregnant rats, chromium, insulin, carnosine, oxidative stress
Abstract
Backgrounda/Aims: To evaluate the effects of chromium, insulin and carnosine supplementation on serum and tissue levels of oxidative stress markers in streptozotocin (STZ)-induced gestational diabetes mellitus (GDM).
Methods: 132 Sprague-Dawley rats divided into eight groups such as control, sham I, sham II, DM, DM+insulin, DM+chromium, DM+carnosine, and DM+chromium plus carnosine. The rats were sacrificed on day 17 of pregnancy to collect samples. Maternal and fetal levels of 8-OHdG, total antioxidant capacity (TAC) and total oxidant status (TOS) were measured. Immunohistochemical staining was used in the analysis of different maternal tissues, such as brain, kidney, liver, heart, ovary and pancreas.
Results: Increased 8-OHdG and TOS and decreased TAC levels were found in the DM group compared to controls (all, p<0.05). Improvements in biochemical markers, 8-OHdG, TAC and TOS were observed in recipients of insulin, chromium, carnosine or chromium and carnosine (all, p<0.05).
Conclusions: Use of chromium, insulin, or carnosine supplementation may alleviate GDM-related oxidative changes and could prevent oxidative stress triggered by glucose use.
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References
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2. Pihelgas A, Prior A, Ospina MB, Jain V. Decreasing Insulin Requirements in Pregnancy With Pre-existing or Gestational Diabetes: Management Practices Across Canada. J Obstet Gynaecol Can. 2021;43:1180-1183.e1
3. Behboudi-Gandevani S, Bidhendi-Yarandi R, Panahi MH, Vaismoradi M. The effect of mild gestational diabetes mellitus treatment on adverse pregnancy outcomes: A systemic review and meta-analysis. Front Endocrinol (Lausanne). 2021;12:271.
4. Association AD. 2. Classification and diagnosis of diabetes: standards of medical care in diabetes—2018. Diabetes Care. 2018;41(Supplement 1):S13-S27.
5. Pathirana MM, Lassi ZS, Ali A, Arstall MA, Roberts CT, Andraweera PH. Association between metabolic syndrome and gestational diabetes mellitus in women and their children: a systematic review and meta-analysis. Endocrine. 2021;71(2):310-320.
6. Yaribeygi H, Atkin SL, Sahebkar A. A review of the molecular mechanisms of hyperglycemia‐induced free radical generation leading to oxidative stress. J Cell Physiol. 2019;234(2):1300-1312.
7. De Mendonça ELSS, Fragoso MBT, De Oliveira JM, Xavier JA, Goulart MOF, De Oliveira ACM. Gestational Diabetes Mellitus: The Crosslink among Inflammation, Nitroxidative Stress, Intestinal Microbiota and Alternative Therapies. Antioxidants. 2022;11(1):129.
8. Artioli GG, Sale C, Jones RL. Carnosine in health and disease. Eur J Sport Sci. 2019;19(1):30-39.
9. Hue J-J, Kim J-S, Kim J-H, et al. Anti-glycemic effect of L-carnosine in streptozotocin-induced diabetic mice. Korean J Vet Res. 2010;50(2):105-111.
10. Houjeghani S, Kheirouri S, Faraji E, Jafarabadi MA. L-Carnosine supplementation attenuated fasting glucose, triglycerides, advanced glycation end products, and tumor necrosis factor–α levels in patients with type 2 diabetes: a double-blind placebo-controlled randomized clinical trial. Nutr Res. 2018;49:96-106.
11. Ghodsi R, Kheirouri S. Carnosine and advanced glycation end products: a systematic review. Amino Acids. 2018;50(9):1177-1186.
12. Khodavirdipour A, Haddadi F, Keshavarzi S. Chromium supplementation; negotiation with diabetes mellitus, hyperlipidemia and depression. J Diabetes Metab Disord. 2020;19(1):585-595.
13. Talab AT, Abdollahzad H, Nachvak SM, et al. Effects of chromium picolinate supplementation on cardiometabolic biomarkers in patients with type 2 diabetes mellitus: A randomized clinical trial. Clin Nutr Res. 2020;9(2):97-106.
14. Erel O. A novel automated method to measure total antioxidant response against potent free radical reactions. Clin Biochem. 2004;37(2):112-119.
15. Schaberg E, Jordan W, Kuyatt BL. Artificial intelligence in automated classification of rat vaginal smear cells. Anal Quant Cytol Histol. 1992;14(6):446-450.
16. Mitchell S, Shaw D. The worldwide epidemic of female obesity. Best Pract Res Clin Obstet Gynaecol. 2015;29(3):289-299.
17. Saravanan P, Magee LA, Banerjee A, et al. Gestational diabetes: opportunities for improving maternal and child health. Lancet Diabetes Endocrinol. 2020;8(9):793-800.
18. Singh R, Farooq SA, Mannan A, et al. Animal models of diabetic microvascular complications: Relevance to clinical features. Biomed Pharmacother. 2022;145:112305.
19. Gröschl M. The physiological role of hormones in saliva. Bioessays. 2009;31(8):843-852.
20. Chera S, Baronnier D, Ghila L, et al. Diabetes recovery by age-dependent conversion of pancreatic δ-cells into insulin producers. Nature. 2014;514(7523):503-507.
21. Kooshki F, Tutunchi H, Vajdi M, et al. A Comprehensive insight into the effect of chromium supplementation on oxidative stress indices in diabetes mellitus: A systematic review. Clin Exp Pharmacol Physiol. 2021;48(3):291-309.
22. Shinde UA, Sharma G, Xu YJ, Dhalla NS, Goyal RK. Insulin sensitising action of chromium picolinate in various experimental models of diabetes mellitus. J Trace Elem Med Biol. 2004;18(1):23-32.
23. Sahin K, Onderci M, Tuzcu M, et al. Effect of chromium on carbohydrate and lipid metabolism in a rat model of type 2 diabetes mellitus: the fat-fed, streptozotocin-treated rat. Metabolism. 2007;56(9):1233-1240.
24. Geyikli İ, Bayıl S. Kromun İnsülin Duyarlılığı İle İlişkisi. Gaziantep Tıp Dergisi. 2008;14:59-63.
25. Dos Santos JM, Tewari S, Mendes RH. The role of oxidative stress in the development of diabetes mellitus and its complications. J Diabetes Res. 2019;2019:4189813.
26. Wang J, Wang H. Oxidative stress in pancreatic beta cell regeneration. Oxid Med Cell Longev. 2017;2017:1930261..
27. Korkmaz KS, Butuner BD, Roggenbuck D. Detection of 8-OHdG as a diagnostic biomarker. J Lab Precis Med. 2018;3:95.
28. Cadet J, Douki T, Gasparutto D, Ravanat J-L. Oxidative damage to DNA: formation, measurement and biochemical features. Mutat Res. 2003;531(1-2):5-23.
29. Alkadi H. A review on free radicals and antioxidants. Disord Drug Targets. 2020;20(1):16-26.
Published
2022-12-26
How to Cite
1.
Yardim M, Aydin S, Kuloglu T. Effect of chromium and L-carnosine use on oxidative stress markers in experimental model of gestational diabetes mellitus. jms [Internet]. 2022Dec.26 [cited 2026Oct.2];25(4):37-6. Available from: https://www.jmsskims.org/index.php/jms/article/view/1219
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